Study of regulation and spatial organization of spermatogenesis in adolescent rat

Author: Irina Modebadze
Co-authors: D. Dzidziguri, M. Mikava, Al. Kordzadze, N. Chaduneli, N. Papunashvili
Keywords: spermatogenesis, protein complex.
Annotation:

Introduction: Spermatogenesis in adult mammals, which lasts for a lifetime, depends on the sperm stem cells. By their asymmetric division is maintained the stem cell population and at the same time arise the progenitor cells produce spermatozoa. Spermatogenesis is regulated mainly by endocrine factors and also by testicular paracrine/autocrine growth factors. However, the regulation process of spermatozoa maturation and differentiation and the molecular mechanism of spermatogenesis wave spread is obscure. Therefore, it is importance to study and use of regulatory factors for treatment of pathologies caused by different disorders. We have previously shown that rats spermatozoa, like other tissues (liver, pancreas, kidney, heart, brain), contain the thermostable protein complex (TPC). By electrophoresis was found that TPC from different tissues are similar to each other. The aim of the work is the study of the growth regulated protein complex from spermatozoa and 3D reconstruction of the adolescent rat testes tubules. Material and Methods: Material - testes, heart and pancreas of adolescent rat; Methods - isolation of TPC by alcohol extraction; Preparation of histological slides from fixed material; Analysis of serial slices in light microscope; Spatial Modeling 3Dmax Program. Result: It has been shown that the TPC from rat’s spermatozoa inhibits proliferation of homotypic and heterotypic tissues in adolescent rats. The proliferative activity of testes we evaluated by the ratio of the spermatogenetically active and passive seminiferous tubules cut sections. With serial slice analysis has been constructed the 12mm segment of the seminiferous tube, in which the size of spermatogenetically active and passive areas were determined. Conclusion: Spermatozoa of white rats, like other tissues, contain thermostable protein complex (TPC) inhibiting cell proliferation. Adolescent rats are characterized by the alternating areas with different activity of spermatogenesis and this alternation can be considered as age peculiarities.