The dependence of properties of prostate tissue galactose-specific lectins on gland pathology

Author: Elene Davitashvili
Co-authors: Elene Davitashvili, Nunu Mickevich, Tamar Tsercvadze, Marine Koshoridze
Keywords: prostate, mitochondrion, cytoplasm, galactose-specific lectins, cell survival, apoptotic cells
Annotation:

Changes of some biochemical characteristics of galactose-specific lectins, separated from post-operative tissue subcellular fractions (cytoplasm and mitochondria) in human prostate healthy (Norma, obtained by cytoprostatactomy) and diseased gland tissue (1.BHP, Benign prostatic hyperplasia; 2.HGPIN, high grade intraepithelial neoplasia; 3. AAH, atypical adenomatous hyperplasia; 4. PC, adenocarcinoma) was studied. The galactose-specific lectin is a 60-kDa protein (dodecyl sulphate-based sampling, electrophoresis in polyacrylamide gel), which was separated from all tasted tissues mitochondrion during comparative analysis, however it was found gal-3, by using of galectin antibodies in mitochondrial fractions of BPH, HGPIN and AAH. Different molecular weight galactose-specific lectins were found in the cytoplasmic fraction of the disease depending form, including galectins -3. It was studied effect of lectins separated and purified by affinity chromatography on survival of MEC1-line lymphocytes in peripheral blood of healthy person and leukemia in model experiments. It was not detected any changes in effect of mitochondrial galactose-specific lectins on survival of healthy lymphocytes. Cytoplasmic galactose-specific lecins, separated from HGPIN and PC was reliably suppresses viability of healthy lymphocytes. MEC1-line lymphocyte viability decreases by cytoplasmic lectins separated from Norma, BPH, and HGPIN PC tissues. The obtained data indicates the involvement of galactose-specific lectins (cytoplasmic fraction) in cell apoptosis process. The role of lectins (PC and HGPIN tissue as a precursor for development of tumors) was also studied in lymphocytes apoptosis. The cell cycles were studied by flow cytometry (fluorescence counterstain, proprium iodide PI) incubation 0 sec, 24 h, 48 h, 72 h, lectin concentration 5 mkg/10X10-4 cell. We were counting preapoptotic and apoptotic cells. According to the optical density, the different effect of lectins was revealed in to cycle duration. Counting of apoptotic cells starts from 0 sec, 24 hours with the influence of PC-diagnosed tissue cytoplasmic lectins, preapoptotic cells formation starts from 48 hours incubation lectins with lymphocytes. In contrast to HGPIN diagnosis tissue lectins prevailed the formation of apoptotic cells. Thus, it is probable that cytoplasmic and mitochondrial galactose-specific lectins of prostate tissue cells involved in the apoptosis process, but their biological properties are dependent on the pathogenesis in contrast to the lectines allocated from healthy tissue. However, their biological properties are dependent on pathogenesis are changing in contrast to the lectins separated from healthy tissue.